APA
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O'Leary, E., Tian, Y., Niğdelioğlu, R., Witt, L., Cetin-Atalay, R., Meliton, A., … Hamanaka, R. (2020). TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-dependent ATF4 Activation. American Journal of Respiratory Cell and Molecular Biology.
Chicago/Turabian
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O'Leary, E., Yufeng Tian, R. Niğdelioğlu, L. Witt, R. Cetin-Atalay, A. Meliton, P. S. Woods, et al. “TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-Dependent ATF4 Activation.” American journal of respiratory cell and molecular biology (2020).
MLA
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O'Leary, E., et al. “TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-Dependent ATF4 Activation.” American Journal of Respiratory Cell and Molecular Biology, 2020.
BibTeX Click to copy
@article{e2020a,
title = {TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-dependent ATF4 Activation.},
year = {2020},
journal = {American journal of respiratory cell and molecular biology},
author = {O'Leary, E. and Tian, Yufeng and Niğdelioğlu, R. and Witt, L. and Cetin-Atalay, R. and Meliton, A. and Woods, P. S. and Kimmig, L. and Sun, K. and Gökalp, G. and Mutlu, G. and Hamanaka, R.}
}
Idiopathic pulmonary fibrosis is a fatal disease characterized by the TGF-β-dependent differentiation of lung fibroblasts into myofibroblasts, leading to excessive deposition of collagen proteins and progressive scarring. We have previously shown that synthesis of collagen by myofibroblasts requires de novo synthesis of glycine, the most abundant amino acid found in collagen protein. TGF-β upregulates the expression of the enzymes of the de novo serine/glycine synthesis pathway in lung fibroblasts; however, the transcriptional and signaling regulators of this pathway remain incompletely understood. Here we demonstrate that TGF-β promotes accumulation of Activating Transcription Factor 4 (ATF4) which is required for increased expression of the serine/glycine synthesis pathway enzymes in response to TGF-β. We found that induction of the Integrated Stress Response (ISR) contributes to TGF-β-induced ATF4 activity; however, the primary driver of ATF4 downstream of TGF-β is activation of the Mechanistic Target of Rapamycin Complex 1 (mTORC1). TGF-β activates the PI3-kinase-Akt-mTOR pathway, and inhibition of PI3-kinase prevents activation of downstream signaling and induction of ATF4. Using a panel of mTOR inhibitors, we found that ATF4 activation is dependent on mTORC1, independent of mTORC2. Rapamycin, which incompletely and allosterically inhibits mTORC1 had no effect on TGF-β-mediated induction of ATF4; however, Rapalink-1, which specifically targets the kinase domain of mTORC1 completely inhibited ATF4 induction and metabolic reprogramming downstream of TGF-β. Our results provide insight into the mechanisms of metabolic reprogramming in myofibroblasts and clarify contradictory published findings on the role of mTOR inhibition in myofibroblast differentiation.
